白头翁汤对大鼠跨内皮迁移的中性粒细胞溶菌酶的影响

doi:10.11843/j.issn.0366-6964.2014.01.020

Abstract

Abstract:

To investigate the antibacteria mechanism of Pulsatilla decoction, the Transwell system was used to establish the model of leukocyte migration induced by Lipopolysaccharide (LPS). The lysozyme concentrations and activity of the polymorph nuclear neutrophils (PMNs), which could pass through the rat intestinal mucosa microvascular endothelial cells (RIMECs) and not, were tested by the means of Agarose rocket electrophoresis and Fluorescence. The results showed that among the transendothelial migration of PMNs, the lysozyme concentrations of the “PD+LPS” group (compared with the control group) and the LPS group (compared with the PD group) significantly increased (P＜0.01), and their lysozyme activity also increased significantly (P＜0.05). There is no significant difference in the groups among the no transendothelial PMNs (P＞0.05). The results indicated that one of the antibacterial mechanism of PD is closely related with the protection against LPS and activation of transendotelial
migration of PMNs lysozyme.

CLC Number:

">R285.5

LIU Xiao-ye, ZHANG Tao, DONG Hong, DUAN Hui-qin, HU Ge, MU Xiang. Effects of Pulsatilla Decoction on the Lysozyme of Transendothelial Migration of Polymorph Nuclear Neutrophils in Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 142-146.

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Effects of Pulsatilla Decoction on the Lysozyme of Transendothelial Migration of Polymorph Nuclear Neutrophils in Rat

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